Translin

Protein-coding gene in the species Homo sapiens

TSN
Available structures
PDBOrtholog search: PDBe RCSB
List of PDB id codes

1J1J, 3PJA, 3QB5, 4WYV

Identifiers
AliasesTSN, BCLF-1, C3PO, RCHF1, REHF-1, TBRBP, TRSLN, translin
External IDsOMIM: 600575; MGI: 109263; HomoloGene: 3397; GeneCards: TSN; OMA:TSN - orthologs
Gene location (Human)
Chromosome 2 (human)
Chr.Chromosome 2 (human)[1]
Chromosome 2 (human)
Genomic location for TSN
Genomic location for TSN
Band2q14.3Start121,737,103 bp[1]
End121,767,853 bp[1]
Gene location (Mouse)
Chromosome 1 (mouse)
Chr.Chromosome 1 (mouse)[2]
Chromosome 1 (mouse)
Genomic location for TSN
Genomic location for TSN
Band1|1 E2.3Start118,222,508 bp[2]
End118,239,469 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • gonad

  • secondary oocyte

  • skin of hip

  • optic nerve

  • skin of thigh

  • ganglionic eminence

  • thoracic diaphragm

  • germinal epithelium

  • islet of Langerhans

  • superficial temporal artery
Top expressed in
  • trigeminal ganglion

  • endothelial cell of lymphatic vessel

  • Rostral migratory stream

  • renal corpuscle

  • primitive streak

  • medullary collecting duct

  • vestibular sensory epithelium

  • neural tube

  • Paneth cell

  • superior cervical ganglion
More reference expression data
BioGPS


More reference expression data
Gene ontology
Molecular function
  • DNA binding
  • nuclease activity
  • endonuclease activity
  • sequence-specific DNA binding
  • protein binding
  • mRNA binding
  • hydrolase activity
  • RNA binding
  • single-stranded DNA binding
  • identical protein binding
  • endoribonuclease activity
  • protein-containing complex binding
Cellular component
  • cytoplasm
  • cytosol
  • nucleus
Biological process
  • DNA recombination
  • nucleic acid phosphodiester bond hydrolysis
  • RNA metabolic process
  • RNA phosphodiester bond hydrolysis, endonucleolytic
  • production of siRNA involved in RNA interference
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

7247

22099

Ensembl

ENSG00000211460

ENSMUSG00000026374

UniProt

Q15631

Q62348

RefSeq (mRNA)

NM_001261401
NM_004622

NM_011650

RefSeq (protein)

NP_001248330
NP_004613

NP_035780

Location (UCSC)Chr 2: 121.74 – 121.77 MbChr 1: 118.22 – 118.24 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Translin is a DNA-binding protein that in humans is encoded by the TSN gene.[5][6][7] Together with translin-associated factor X, translin forms the component 3 of promoter of RISC (C3PO) complex which facilitates endonucleolytic cleavage of the passenger strand during microRNA loading into the RNA-induced silencing complex (RISC).[8][9][10]

Function

This gene encodes a DNA-binding protein which specifically recognizes conserved target sequences at the breakpoint junction of chromosomal translocations. Translin polypeptides form a multimeric structure that is responsible for its DNA-binding activity. Recombination-associated motifs and translin-binding sites are present at recombination hotspots and may serve as indicators of breakpoints in genes which are fused by translocations. These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms.[7]

Interactions

Translin has been shown to interact with PPP1R15A.[11]

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000211460 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000026374 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Kasai M, Aoki K, Matsuo Y, Minowada J, Maziarz RT, Strominger JL (July 1994). "Recombination hotspot associated factors specifically recognize novel target sequences at the site of interchromosomal rearrangements in T-ALL patients with t(8;14)(q24;q11) and t(1;14)(p32;q11)". International Immunology. 6 (7): 1017–25. doi:10.1093/intimm/6.7.1017. PMID 7947454.
  6. ^ Aoki K, Inazawa J, Takahashi T, Nakahara K, Kasai M (July 1997). "Genomic structure and chromosomal localization of the gene encoding translin, a recombination hotspot binding protein". Genomics. 43 (2): 237–41. doi:10.1006/geno.1997.4796. PMID 9244443.
  7. ^ a b "Entrez Gene: TSN translin".
  8. ^ Liu Y, Ye X, Jiang F, Liang C, Chen D, Peng J, Kinch LN, Grishin NV, Liu Q (August 2009). "C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation". Science. 325 (5941): 750–3. Bibcode:2009Sci...325..750L. doi:10.1126/science.1176325. PMC 2855623. PMID 19661431.
  9. ^ Ye X, Huang N, Liu Y, Paroo Z, Huerta C, Li P, Chen S, Liu Q, Zhang H (June 2011). "Structure of C3PO and mechanism of human RISC activation". Nature Structural & Molecular Biology. 18 (6): 650–7. doi:10.1038/nsmb.2032. PMC 3109212. PMID 21552258.
  10. ^ Ha M, Kim VN (August 2014). "Regulation of microRNA biogenesis". Nature Reviews Molecular Cell Biology. 15 (8): 509–24. doi:10.1038/nrm3838. PMID 25027649. S2CID 205495632.
  11. ^ Hasegawa T, Isobe K (August 1999). "Evidence for the interaction between Translin and GADD34 in mammalian cells". Biochimica et Biophysica Acta (BBA) - General Subjects. 1428 (2–3): 161–8. doi:10.1016/s0304-4165(99)00060-4. PMID 10434033.

Further reading

  • Aoki K, Suzuki K, Sugano T, Tasaka T, Nakahara K, Kuge O, Omori A, Kasai M (June 1995). "A novel gene, Translin, encodes a recombination hotspot binding protein associated with chromosomal translocations". Nature Genetics. 10 (2): 167–74. doi:10.1038/ng0695-167. PMID 7663511. S2CID 9678546.
  • Maruyama K, Sugano S (January 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
  • Aoki K, Nakahara K, Ikegawa C, Seto M, Takahashi T, Minowada J, Strominger JL, Maziarz RT, Kasai M (April 1994). "Nuclear proteins binding to a novel target sequence within the recombination hotspot regions of Bcl-2 and the immunoglobulin DH gene family". Oncogene. 9 (4): 1109–15. PMID 8134113.
  • Aoki K, Ishida R, Kasai M (January 1997). "Isolation and characterization of a cDNA encoding a Translin-like protein, TRAX". FEBS Letters. 401 (2–3): 109–12. doi:10.1016/S0014-5793(96)01444-5. PMID 9013868. S2CID 46704829.
  • Kasai M, Matsuzaki T, Katayanagi K, Omori A, Maziarz RT, Strominger JL, Aoki K, Suzuki K (April 1997). "The translin ring specifically recognizes DNA ends at recombination hot spots in the human genome". The Journal of Biological Chemistry. 272 (17): 11402–7. doi:10.1074/jbc.272.17.11402. PMID 9111049.
  • Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (October 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
  • Hasegawa T, Isobe K (August 1999). "Evidence for the interaction between Translin and GADD34 in mammalian cells". Biochimica et Biophysica Acta (BBA) - General Subjects. 1428 (2–3): 161–8. doi:10.1016/s0304-4165(99)00060-4. PMID 10434033.
  • Badge RM, Yardley J, Jeffreys AJ, Armour JA (May 2000). "Crossover breakpoint mapping identifies a subtelomeric hotspot for male meiotic recombination". Human Molecular Genetics. 9 (8): 1239–44. doi:10.1093/hmg/9.8.1239. PMID 10767349.
  • Hosaka T, Kanoe H, Nakayama T, Murakami H, Yamamoto H, Nakamata T, Tsuboyama T, Oka M, Kasai M, Sasaki MS, Nakamura T, Toguchida J (November 2000). "Translin binds to the sequences adjacent to the breakpoints of the TLS and CHOP genes in liposarcomas with translocation t(12;6)". Oncogene. 19 (50): 5821–5. doi:10.1038/sj.onc.1203943. hdl:2433/150205. PMID 11126370.
  • Chennathukuzhi VM, Kurihara Y, Bray JD, Hecht NB (April 2001). "Trax (translin-associated factor X), a primarily cytoplasmic protein, inhibits the binding of TB-RBP (translin) to RNA". The Journal of Biological Chemistry. 276 (16): 13256–63. doi:10.1074/jbc.M009707200. PMID 11278549.
  • Lee SP, Fuior E, Lewis MS, Han MK (November 2001). "Analytical ultracentrifugation studies of translin: analysis of protein-DNA interactions using a single-stranded fluorogenic oligonucleotide". Biochemistry. 40 (46): 14081–8. doi:10.1021/bi010302t. PMID 11705401.
  • Erdemir T, Bilican B, Oncel D, Goding CR, Yavuzer U (January 2002). "DNA damage-dependent interaction of the nuclear matrix protein C1D with Translin-associated factor X (TRAX)". Journal of Cell Science. 115 (Pt 1): 207–16. doi:10.1242/jcs.115.1.207. hdl:11693/24740. PMID 11801738.
  • Han MK, Lin P, Paek D, Harvey JJ, Fuior E, Knutson JR (March 2002). "Fluorescence studies of pyrene maleimide-labeled translin: excimer fluorescence indicates subunits associate in a tail-to-tail configuration to form octamer". Biochemistry. 41 (10): 3468–76. doi:10.1021/bi015901e. PMID 11876655.
  • Sengupta K, Rao BJ (December 2002). "Translin binding to DNA: recruitment through DNA ends and consequent conformational transitions". Biochemistry. 41 (51): 15315–26. doi:10.1021/bi026378m. PMID 12484770.
  • Li K, Wang L, Cheng J, Lu YY, Zhang LX, Mu JS, Hong Y, Liu Y, Duan HJ, Wang G, Li L, Chen JM (February 2003). "Interaction between hepatitis C virus core protein and translin protein--a possible molecular mechanism for hepatocellular carcinoma and lymphoma caused by hepatitis C virus". World Journal of Gastroenterology. 9 (2): 300–3. doi:10.3748/wjg.v9.i2.300. PMC 4611333. PMID 12532453.
  • Yang S, Cho YS, Chennathukuzhi VM, Underkoffler LA, Loomes K, Hecht NB (March 2004). "Translin-associated factor X is post-transcriptionally regulated by its partner protein TB-RBP, and both are essential for normal cell proliferation". The Journal of Biological Chemistry. 279 (13): 12605–14. doi:10.1074/jbc.M313133200. PMID 14711818.

External links

  • Overview of all the structural information available in the PDB for UniProt: Q15631 (Human Translin) at the PDBe-KB.
  • Overview of all the structural information available in the PDB for UniProt: Q62348 (Mouse Translin) at the PDBe-KB.
  • v
  • t
  • e
  • 1j1j: Crystal Structure of human Translin
    1j1j: Crystal Structure of human Translin
  • 1key: Crystal Structure of Mouse Testis/Brain RNA-binding Protein (TB-RBP)
    1key: Crystal Structure of Mouse Testis/Brain RNA-binding Protein (TB-RBP)


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